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vascular smooth muscle cells vsmcs  (ATCC)


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    ATCC vascular smooth muscle cells vsmcs
    Vascular Smooth Muscle Cells Vsmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+vsmcs/Primary+Aortic+Smooth+Muscle+Cells%3B+Normal%2C+Human/pm41906524-31-0-13
    Average 95 stars, based on 391 article reviews
    vascular smooth muscle cells vsmcs - by Bioz Stars, 2026-10
    95/100 stars

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    Cell Culture:

    Article Title: Downregulation of FLT1 delays vascular aging by suppressing MAPK signaling pathway activation in vascular smooth muscle cells​.
    Article Snippet: Vascular aging is a critical pathological basis of cardiovascular diseases (CVDs), involving functional abnormalities of vascular smooth muscle cells (VSMCs).. This study investigated whether downregulation of Fmsrelated tyrosine kinase 1 (FLT1) ameliorates vascular aging by inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway in VSMCs.. Angiotensin II (Ang II) and bleomycin (Bleo) were used to induce VSMC senescence in vitro and establish a mouse aortic aging model in vivo.

    Article Title: Ultrasmall Cu 2-x Se nanoparticles alleviate vascular calcification through inhibiting oxidative stress and NF-κB/NLRP3-mediated inflammation.
    Article Snippet: .. Human VSMCs (CRL-1999, ATCC, USA) were cultured under identical conditions. ..

    Article Title: Porous Materials with Water‐Triggered Instant Transformation to Robust Hydrogel Adhesives for Rapid Hemostasis of High‐Pressure Hemorrhages
    Article Snippet: Uncontrolled traumatic hemorrhage is a leading cause of preventable death.. As two main forms of hemostatic materials, however, both sponges and hydrogel adhesives usually fail to control massive hemorrhage due to their respective shortcomings.. Hemostatic sponge shows weak tissue adhesion, while the gelation process of hydrogel makes it difficult to seal high-pressure bleeding wounds.

    Article Title: Ultrasmall Cu 2−x Se nanoparticles alleviate vascular calcification through inhibiting oxidative stress and NF-κB/NLRP3-mediated inflammation
    Article Snippet: .. Human VSMCs (CRL-1999, ATCC, USA) were cultured under identical conditions. ..

    Article Title: Mechanical stress accelerates vascular calcification by Piezo1/BMP2 of vascular smooth muscle cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: A novel protein B2URF3 from Akkermansia muciniphila increased by intermittent fasting alleviates vascular calcification
    Article Snippet: Mice were euthanized by a single intraperitoneal injection of sodium pentobarbital (200 mg/kg), the aorta of mice was collected for Alizarin Red S staining (ARS) and calcium content assay. .. Human VSMCs (ATCC, CRL-1999) were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 containing 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin, and cells at passages 3 to 8 were used for subsequent experiments. .. To induce VSMC calcification, A kk -EVs and several candidate proteins were added to the osteogenic culture medium (HUXMX-90021, Cyagen Biosciences) during cell intervention.

    Modification:

    Article Title: Downregulation of FLT1 delays vascular aging by suppressing MAPK signaling pathway activation in vascular smooth muscle cells​.
    Article Snippet: Vascular aging is a critical pathological basis of cardiovascular diseases (CVDs), involving functional abnormalities of vascular smooth muscle cells (VSMCs).. This study investigated whether downregulation of Fmsrelated tyrosine kinase 1 (FLT1) ameliorates vascular aging by inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway in VSMCs.. Angiotensin II (Ang II) and bleomycin (Bleo) were used to induce VSMC senescence in vitro and establish a mouse aortic aging model in vivo.

    Article Title: Porous Materials with Water‐Triggered Instant Transformation to Robust Hydrogel Adhesives for Rapid Hemostasis of High‐Pressure Hemorrhages
    Article Snippet: Uncontrolled traumatic hemorrhage is a leading cause of preventable death.. As two main forms of hemostatic materials, however, both sponges and hydrogel adhesives usually fail to control massive hemorrhage due to their respective shortcomings.. Hemostatic sponge shows weak tissue adhesion, while the gelation process of hydrogel makes it difficult to seal high-pressure bleeding wounds.

    Article Title: A novel protein B2URF3 from Akkermansia muciniphila increased by intermittent fasting alleviates vascular calcification
    Article Snippet: Mice were euthanized by a single intraperitoneal injection of sodium pentobarbital (200 mg/kg), the aorta of mice was collected for Alizarin Red S staining (ARS) and calcium content assay. .. Human VSMCs (ATCC, CRL-1999) were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 containing 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin, and cells at passages 3 to 8 were used for subsequent experiments. .. To induce VSMC calcification, A kk -EVs and several candidate proteins were added to the osteogenic culture medium (HUXMX-90021, Cyagen Biosciences) during cell intervention.

    Incubator:

    Article Title: Porous Materials with Water‐Triggered Instant Transformation to Robust Hydrogel Adhesives for Rapid Hemostasis of High‐Pressure Hemorrhages
    Article Snippet: Uncontrolled traumatic hemorrhage is a leading cause of preventable death.. As two main forms of hemostatic materials, however, both sponges and hydrogel adhesives usually fail to control massive hemorrhage due to their respective shortcomings.. Hemostatic sponge shows weak tissue adhesion, while the gelation process of hydrogel makes it difficult to seal high-pressure bleeding wounds.

    other:

    Article Title: Exploration of Conserved Human Adipose Subpopulations Using Targeted Single‐Nuclei RNA Sequencing Data Sets
    Article Snippet: Immunofluorescence staining was performed using antibodies detailed in Table , including those detecting cluster of differentiation (CD) 31 (Cell Marque, Rocklin, CA), chondroitin sulfate proteoglycan 4 (NG2; Abcam, Waltham, MA), PDGFRB (Cell Signaling Technology, Danvers, MA [n=3]), RGS5 (Invitrogen, Waltham, MA [n=3]), transient receptor potential cation channel subfamily C member 6 (TRPC6; Proteintech, Chicago, IL [n=3]), ATP binding cassette subfamily C member 9 (ABCC9; LSBio, Newark, CA [n=3]), or COL25A1 (LSBio [n=4]) at 4°C overnight, followed by goat fluorescein isothiocyanate–anti‐mouse IgG (Vector Laboratories, Newark, CA) and Alexa fluor 647–anti‐rabbit IgG (Molecular Probes, Eugene, OR) or fluorescein isothiocyanate–anti‐rabbit IgG (Vector Laboratories) and donkey Rhodamine X‐anti‐mouse immunoglobulin G (Jackson ImmunoResearch Laboratories, West Grove, PA).



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    Comparison of baseline NFR2 protein levels ( A ) NFR2 activity ( B ) and NRF2 transcript expression without additional oxidative stress induction. ( A ) NRF2 protein levels were determined by ELISA and normalized to the total protein level of each sample. Data are shown as pg NRF2 per µg protein. Each color circle represents a normalized value derived from n = 4 AAA-SMC, n = 3 healthy <t>VSMC</t> samples and one immortalized cell line (IM), analysed in duplicate. ( B ) NRF2 binding activity to DNA was assayed from nuclear extracts by using a colorimetric NRF2 activity assay. Data were normalized to the protein content of the corresponding nuclear extracts. Each color circle represents a normalized value derived from n = 2 AAA-SMC, n = 3 healthy VSMC and one immortalized cell line (IM), analysed in duplicate. ( C ) NFE2L2 transcript expression was analysed by qPCR using specific primers. Data show the 2 −(ΔCt) values. Each colour circle represents a normalized value of n = 2 AAA-SMC (AAA), n = 3 healthy VSMC (healthy), and one immortalized cell line (IM), analysed in triplicate. Bars represent the mean ± SEM. Data were statistically analysed by ordinary one-way ANOVA and Tukey’s multiple comparison test; ns: not significant. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    The effect of TCC on the biological function <t>of</t> <t>vascular</t> smooth muscle cells <t>(VSMCs).</t> (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.
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    Image Search Results


    Comparison of baseline NFR2 protein levels ( A ) NFR2 activity ( B ) and NRF2 transcript expression without additional oxidative stress induction. ( A ) NRF2 protein levels were determined by ELISA and normalized to the total protein level of each sample. Data are shown as pg NRF2 per µg protein. Each color circle represents a normalized value derived from n = 4 AAA-SMC, n = 3 healthy VSMC samples and one immortalized cell line (IM), analysed in duplicate. ( B ) NRF2 binding activity to DNA was assayed from nuclear extracts by using a colorimetric NRF2 activity assay. Data were normalized to the protein content of the corresponding nuclear extracts. Each color circle represents a normalized value derived from n = 2 AAA-SMC, n = 3 healthy VSMC and one immortalized cell line (IM), analysed in duplicate. ( C ) NFE2L2 transcript expression was analysed by qPCR using specific primers. Data show the 2 −(ΔCt) values. Each colour circle represents a normalized value of n = 2 AAA-SMC (AAA), n = 3 healthy VSMC (healthy), and one immortalized cell line (IM), analysed in triplicate. Bars represent the mean ± SEM. Data were statistically analysed by ordinary one-way ANOVA and Tukey’s multiple comparison test; ns: not significant. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Cells

    Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

    doi: 10.3390/cells15161499

    Figure Lengend Snippet: Comparison of baseline NFR2 protein levels ( A ) NFR2 activity ( B ) and NRF2 transcript expression without additional oxidative stress induction. ( A ) NRF2 protein levels were determined by ELISA and normalized to the total protein level of each sample. Data are shown as pg NRF2 per µg protein. Each color circle represents a normalized value derived from n = 4 AAA-SMC, n = 3 healthy VSMC samples and one immortalized cell line (IM), analysed in duplicate. ( B ) NRF2 binding activity to DNA was assayed from nuclear extracts by using a colorimetric NRF2 activity assay. Data were normalized to the protein content of the corresponding nuclear extracts. Each color circle represents a normalized value derived from n = 2 AAA-SMC, n = 3 healthy VSMC and one immortalized cell line (IM), analysed in duplicate. ( C ) NFE2L2 transcript expression was analysed by qPCR using specific primers. Data show the 2 −(ΔCt) values. Each colour circle represents a normalized value of n = 2 AAA-SMC (AAA), n = 3 healthy VSMC (healthy), and one immortalized cell line (IM), analysed in triplicate. Bars represent the mean ± SEM. Data were statistically analysed by ordinary one-way ANOVA and Tukey’s multiple comparison test; ns: not significant. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

    Techniques: Comparison, Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Binding Assay

    Dose response of AAA-SMC ( A ), healthy VSMC ( B ), and iHAoSMC ( C ) to acute oxidative stress induced by H 2 O 2 . Cells were exposed to different concentrations of H 2 O 2 for 30 min and analysed for viability using a WST-1 viability assay after recovery times of 0 h, 2 h, 4 h, and 24 h in smooth muscle cell growth medium without H 2 O 2 . Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ****: p < 0.0001.

    Journal: Cells

    Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

    doi: 10.3390/cells15161499

    Figure Lengend Snippet: Dose response of AAA-SMC ( A ), healthy VSMC ( B ), and iHAoSMC ( C ) to acute oxidative stress induced by H 2 O 2 . Cells were exposed to different concentrations of H 2 O 2 for 30 min and analysed for viability using a WST-1 viability assay after recovery times of 0 h, 2 h, 4 h, and 24 h in smooth muscle cell growth medium without H 2 O 2 . Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ****: p < 0.0001.

    Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

    Techniques: Viability Assay, Comparison

    Response of AAA-SMC, immortalized iHAoSMC, and healthy VSMC against acute oxidative stress induced by exposure to H 2 O 2 . ( A ) Treatment scheme for induction of acute oxidative stress. Cell lines were treated with 10 nM of MitoQ or vehicle for 7 days before they were challenged with 100 µM H 2 O 2 for 30 min. ( B ) NRF2 binding activity to DNA (antioxidative response element, ARE). Nuclear extracts were harvested immediately after H 2 O 2 treatment and analysed by using an NRF2 activity assay. ( C ) Transcriptional expression of the NRF2 target gene HMOX1 (HO1), 6 h after H 2 O 2 treatment. Data show the 2 −ΔΔCt values derived from qPCR. ( D ) HO1 protein expression was analysed by ELISA. Protein extracts were harvested after 24 h of regeneration. ( B – D ) Data are derived from n = 2 individual AAA-SMC cultures, n = 3 individual healthy VSMC cell cultures, and one iHAoSMC line. Bars represent the mean ± SEM. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001, ****: p < 0.0001.

    Journal: Cells

    Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

    doi: 10.3390/cells15161499

    Figure Lengend Snippet: Response of AAA-SMC, immortalized iHAoSMC, and healthy VSMC against acute oxidative stress induced by exposure to H 2 O 2 . ( A ) Treatment scheme for induction of acute oxidative stress. Cell lines were treated with 10 nM of MitoQ or vehicle for 7 days before they were challenged with 100 µM H 2 O 2 for 30 min. ( B ) NRF2 binding activity to DNA (antioxidative response element, ARE). Nuclear extracts were harvested immediately after H 2 O 2 treatment and analysed by using an NRF2 activity assay. ( C ) Transcriptional expression of the NRF2 target gene HMOX1 (HO1), 6 h after H 2 O 2 treatment. Data show the 2 −ΔΔCt values derived from qPCR. ( D ) HO1 protein expression was analysed by ELISA. Protein extracts were harvested after 24 h of regeneration. ( B – D ) Data are derived from n = 2 individual AAA-SMC cultures, n = 3 individual healthy VSMC cell cultures, and one iHAoSMC line. Bars represent the mean ± SEM. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001, ****: p < 0.0001.

    Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

    Techniques: Binding Assay, Activity Assay, Expressing, Derivative Assay, Enzyme-linked Immunosorbent Assay, Comparison

    ROS production in AAA-SMC, immortalized iHAoSMC, and in healthy VSMC. Cells were exposed to acute oxidative stress by H 2 O 2 for 30 min, and images were taken after 24 h of regeneration. ( A ) Representative images of ROS staining from a AAA-SMC line. ROS production was examined via CellROX fluorescent dye; nuclei were stained with NucBlue. ( B ) Quantification of ROS fluorescence intensity, normalized to NucBlue fluorescence. Data are derived from n = 2 individual AAA-SMC cultures analysed fivefold ( left panel ) from n = 3 individual healthy VSMC cell cultures analysed in triplicate ( middle panel ) and an iHAoSMC line analysed in six technical replicas ( right panel ). Box plots show the median, maximum, and minimum of values derived from the ROS assay. Each colour circle represents an individual value after normalization. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001.

    Journal: Cells

    Article Title: MitoQ Has Diverse Effects on H 2 O 2 -Induced Oxidative Stress and the NRF2 Signalling Pathway in Aortic Smooth Muscle Cells of Different Origins

    doi: 10.3390/cells15161499

    Figure Lengend Snippet: ROS production in AAA-SMC, immortalized iHAoSMC, and in healthy VSMC. Cells were exposed to acute oxidative stress by H 2 O 2 for 30 min, and images were taken after 24 h of regeneration. ( A ) Representative images of ROS staining from a AAA-SMC line. ROS production was examined via CellROX fluorescent dye; nuclei were stained with NucBlue. ( B ) Quantification of ROS fluorescence intensity, normalized to NucBlue fluorescence. Data are derived from n = 2 individual AAA-SMC cultures analysed fivefold ( left panel ) from n = 3 individual healthy VSMC cell cultures analysed in triplicate ( middle panel ) and an iHAoSMC line analysed in six technical replicas ( right panel ). Box plots show the median, maximum, and minimum of values derived from the ROS assay. Each colour circle represents an individual value after normalization. Data were statistically analysed by two-way ANOVA and Tukey’s multiple comparison test; ns: not significant, *: p < 0.05, ** p < 0.01, ***: p < 0.001.

    Article Snippet: The immortalized VSMC line (iHAoSMC) was obtained from InnoProt ( P10456 -IM, Derio, Spain).

    Techniques: Staining, Fluorescence, Derivative Assay, ROS Assay, Comparison

    The effect of TCC on the biological function of vascular smooth muscle cells (VSMCs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Research and development of novel embolization materials and study on their feasibility of preventing T2EL after EVAR abdominal aortic aneurysm

    doi: 10.3389/fcvm.2026.1740816

    Figure Lengend Snippet: The effect of TCC on the biological function of vascular smooth muscle cells (VSMCs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Article Snippet: Human aortic vascular smooth muscle cells (VSMCs) and human aortic endothelial cells (VECs) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. All cells were primary cells and used between passages 3–8.

    Techniques: CCK-8 Assay, Migration, Control

    The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Research and development of novel embolization materials and study on their feasibility of preventing T2EL after EVAR abdominal aortic aneurysm

    doi: 10.3389/fcvm.2026.1740816

    Figure Lengend Snippet: The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Article Snippet: Human aortic vascular smooth muscle cells (VSMCs) and human aortic endothelial cells (VECs) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. All cells were primary cells and used between passages 3–8.

    Techniques: CCK-8 Assay, Migration, Control